Purification and Characterization of Pea Cytosolic Ascorbate Peroxidase
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چکیده
منابع مشابه
Characterization and crystallization of recombinant pea cytosolic ascorbate peroxidase.
An Escherichia coli expression system has been developed for pea cytosolic ascorbate peroxidase (APX). The enzyme was expressed as a fusion product with the E. coli maltose-binding protein for rapid, affinity chromatography purification. Recombinant ascorbate peroxidase (rAPX) was purified by tryptic digestion to separate the maltose-binding protein from rAPX followed by three chromatographic s...
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Reduced oxygen intermediates are produced in plant tissues from mitochondrial and chloroplastic electron transport pathways and from electron-saturated photosynthetic pigments (Bowler et al., 1992). For instance, the Mehler reaction is a primary source of reduced oxygen intermediates in chloroplasts in which dioxygen is reduced by Fd on the reducing side of PSI to produce .O2-. The dismutation ...
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Switchgrass (Panicum virgatum L.) is being developed as a bioenergy species. Recently an early version of its genome has been released permitting a route to the cloning and analysis of key proteins. Ascorbate peroxidases (APx) are an important part of the antioxidant defense system of plant cells and present a well studied model to understand structure-function relationships. Analysis of the ge...
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Glutathione reductase (EC 1.6.4.2) was purified from intact pea (Pisum sativum) chloroplasts by a method which includes affinity chromatography on ADP-agarose. Fractions from the affinity column which had glutathione reductase activity consisted of polypeptides of 60 and 32 kilodaltons. Separation of the proteins by electrophoresis on native gels showed that glutathione reductase activity was a...
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Acetone fractionated peroxidase from crude extract of Brassica oleracea leaves (Cabbage) was purified in three steps on chromatographic columns, using Sp-Sepharose, DEAE-Sepharose and Con A-Sepharose. The specific activity of purified main isoenzyme (BOC-POD) is 1887 u/mg protein with RZ: 3.1, which is 172 times more than the RZ of crude extract with 4.3% recovery. The molecular weight of BOC-P...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1991
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.97.3.962